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KMID : 0613820030130030291
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Journal of Life Science
2003 Volume.13 No. 3 p.291 ~ p.297
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Identification and phylogenetic analysis of the human endogenous retrovirus HERV-W pol in cDNA library of human fetal brain
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Kim Hee-Soo
Lee Ju-Mi
Jeon Seung-Heui
Kim Tae-Hyung
Lee Won-Ho
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Abstract
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A human endogenous retroviral family (HERV-W) has recently been described that is related to multiple sclerosis- associated retrovirus (MSRV) sequences that have been identified in particles recovered from monocyte cultures from patients with multiple sclerosis. Two pol fragments (HWP-FB10 and HWP-FB12) of HERV-W family were identified and analysed by the PCR approach with cDNA library of human fetal brain. They showed 89 percent nucleotide sequence similarity with that of the HERV-W (accession no. AF009668). Deletion/insertion or point mutation in the coding region of the pol fragments from human fetal brain resulted in amino acid frameshift that induced a mutated protein. Phylogenetic analysis of the HERV-W family from GenBank database indicates that the HWP-FB10 is very closely related to the AC000064 derived from human chromosome 7q21-q22. Further studies on the genetic relationship with neighbouring genes and functional role of these new HERV-W pol sequences are indicated.
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KEYWORD
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cDNA library, Fetal brain, HERV-W, pol fragments, Phylogeny
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